Need some help.
We are trying to get a plasmid into a clinical isolate of E coli.
Transformation does not work, so we have tried electroporation,
conjugation using different methods. We can get pBR328 in but not
a RK6 based plasmid. The control using DH5 work. My first thought
was that maybe it had to do with the oriR of the second plasmid
Any ideas?
Jim
