How to get a plasmid into WT E.coli

jim rogers rogers at usuhs.usuhs.mil
Sat Mar 2 10:57:04 EST 1996

Need some help.

We are trying to get a plasmid into a clinical isolate of E coli.
Transformation does not work, so we have tried electroporation,
conjugation using different methods.  We can get pBR328 in but not
a RK6 based plasmid.  The control using DH5 work.  My first thought
was that maybe it had to do with the oriR of the second plasmid

Any ideas?


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