Hi there.
We are doing some HPLC analysis on culture filtrate from fermentor. We are
using Biorad Aminex HPX-47H column with 0,005M sulfuric acid as mobil fase,
0.6ml/min flow, 100 bar pressure, 45 degrees C, 10 ul injection.
Our problem is bad separation of succinate and lactate peaks. Does someone
have a suggesion on how to improve this resolution/separation?
Thanks,
Trond Moretro,
MATFORSK, Norwegian Food Research Institute
Oslov. 1,1430 Aas, Norway
tel: 64970216 (office) 64970229 (lab.)
Fax: 64970333
E. Mail: Trond.Moretro at matforsk.nlh.no
Forever Peace!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
