In article <4h9r4g$rjn at info.usuhs.mil>, jim rogers
<rogers at usuhs.usuhs.mil> writes
>Need some help.
>>We are trying to get a plasmid into a clinical isolate of E coli.
>Transformation does not work, so we have tried electroporation,
>conjugation using different methods. We can get pBR328 in but not
>a RK6 based plasmid. The control using DH5 work. My first thought
>was that maybe it had to do with the oriR of the second plasmid
>Many clinical E.coli's have type II RM systems both plasmid and Xsomally
carried. It may be that your pBR328 hasn't any RE sites whilst the RK6
derivative has.
Duncan
GeneSys Ltd.
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