How to get a plasmid into WT E.coli

Dr. Duncan Clark duncan at genesys.demon.co.uk
Mon Mar 4 04:35:39 EST 1996

In article <4h9r4g$rjn at info.usuhs.mil>, jim rogers
<rogers at usuhs.usuhs.mil> writes
>Need some help.
>We are trying to get a plasmid into a clinical isolate of E coli.
>Transformation does not work, so we have tried electroporation,
>conjugation using different methods.  We can get pBR328 in but not
>a RK6 based plasmid.  The control using DH5 work.  My first thought
>was that maybe it had to do with the oriR of the second plasmid
Many clinical E.coli's have type II RM systems both plasmid and Xsomally
carried. It may be that your pBR328 hasn't any RE sites whilst the RK6
derivative has.

GeneSys Ltd.
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net