Dear Erma;
Two things that you can do; use larger combs so that the well will be
larger even with shrinkage or you can continue to add stacking gel for a
while to increase the size of you wells as the stacking gel polymerizes
and shrinks.
Good luck.
Michael
******************************************************************************
Michael P. Kolotila, Ph.D. * e-mail: mkolotila at necc.mass.edu
*
Biotechnology Program Coordinator *
Department of Natural Science * voice : 508-374-3887
Northern Essex Community College *
100 Elliott Way * fax : 508-374-3723
Haverhill, MA 01830-2399 *
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On 1 Mar 1996, Erma Lai wrote:
> I have a problem when I prepare the stacking gel. I found my separating
> gel polymerized very well but the stacking gel didn't. The problem is that
> the wells of the stacking gels were shrinked after polymerized. Therefore,
> I only can load small amounts of the samples. Hope someone will help me!
>> Erma
>>
