On Thu, 07 Mar 1996 08:33:05 -0600, sims at acs.ucalgary.ca (Kurt Sims)
wrote:
>In article <4hkoco$s0i at milo.mcs.anl.gov>, Mark Fuller
><mark_fuller at qmgate.anl.gov> wrote:
>>> Can someone tell me what proteose peptone is? Is it like pancreatic
>> digest of casein or something. I don't have a Difco manual or I'd look
>> it up myself. I'm want ot know if a media recipe containing proteose
>> peptone can be substituted with something else.
>>>> Thanks
>>>> Mark Fuller, Ph.D.
>>All I could find is that it is a peptic digest of animal tissue. There
>are three different ones from Difco. Proteose Peptone Difco, Proteose
>Peptone No.2 Difco and Proteose Peptone No.3 Difco.
>>According to the manual No.3 is the best of the three for nutrition in
>culturing fastidious organisms.
>>Sorry, I don't know of any substitutes for it.
>>Kurt
Mark,
I have successfully substituted 1% yeast autolysate with 1% tryptic
digest of casein. The only time that I would not suggest
this substitution is when following a procedure for toxin production.
R.Randolph
--
Robert Randolph Randolph Biomedical
21 McElroy Street West Warwick, RI 02893
401-826-1407 rand777 at ids.nethttp://www2.ids.net/~rand777/home.htm
