We are working with an adenovirus that is known to replicate well in the
continuous cell line HEK-293. However, it doesn't appear to grow well in
HeLa cells. CPE is evident in the HEK-293 cell monolayers, but there is a
block in release of progeny virions and restriction to a single cycle
of infection in the HeLa cells.
We are attempting to compare the replication kinetics between the two
cell lines and are in search of the appropriate protocol (specifically,
the infection conditions, sampling times, method of harvesting progeny
virus, and method of measuring progeny yield). If you are
aware of any protocols appropriately dealing with such an experiment or
you have any ideas, please pass on the information as soon as possible.
Thank you for your time,
Gerard and Neal
Please e-mail to: g.fernandes at utoronto.ca
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