electroporation protocol?

"franco pagotto ", grad student g483190 at LABSUN1.MED.UOTTAWA.CA
Tue Mar 12 18:27:36 EST 1996

Does anyone have a quick method for preparing electroporation cells (E. 
coli) in 50 ml or less, starting from a colony?  

When we prepare cells for storage, we grow them in 500 ml and concentrate 
to .75 ml (10EE10 cells/ml) and use 40 ul.  Thanks for your help and advice.

Ciao for now,


More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net