Keith Haddad writes:
> I am using 1% Hexadecylrimethylammonium bromide as precipitant to
> detect microbes producing pectinase.
> Is there any substitute reagents??
> Is it the bromide that reacts with the pectinase??
> So can I use other bromide compounds??
I may be all wrong but...
I believe you mean 1% Hexadexyltrimethylammonium bromide. And I
believe it is precipitating the pectin. If pectinase is present,
there is no pectin and thus no precipitate. You don't say why you are
looking for a substitute. I'm guessing that the reaction is weak.
I've done work with hyaluronic acid (HA) and hyaluronidase using 1%
hexadexyltrimethylammonium bromide to precipitate the HA. The
reaction is weak. The addition of albumin can strengthen the reaction
but can give you false positives (no precipitation) if there is a
protease present.
Dick Murphy
PS it's not the bromide, hexadexyltrimethylammonium bromide is a
quaternary ammonium compound. You also might try another quat.
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