Hexadecylrimethylammonium bromide substitute??

Richard A. Murphy U14663 at UICVM.UIC.EDU
Mon Mar 11 21:06:23 EST 1996

Keith Haddad writes:
> I am using 1% Hexadecylrimethylammonium bromide  as precipitant to
> detect microbes producing pectinase.
> Is there any substitute reagents??
> Is it the bromide that reacts with the pectinase??
> So can I use other bromide compounds??
I may be all wrong but...
I believe you mean 1% Hexadexyltrimethylammonium bromide.  And I
believe it is precipitating the pectin.  If pectinase is present,
there is no pectin and thus no precipitate.  You don't say why you are
looking for a substitute.  I'm guessing that the reaction is weak.
I've done work with hyaluronic acid (HA) and hyaluronidase using 1%
hexadexyltrimethylammonium bromide to precipitate the HA.  The
reaction is weak.  The addition of albumin can strengthen the reaction
but can give you false positives (no precipitation) if there is a
protease present.
Dick Murphy
PS it's not the bromide, hexadexyltrimethylammonium bromide is a
quaternary ammonium compound.  You also might try another quat.
|Richard A. Murphy, PhD              Bitnet: U14663 at uicvm.bitnet    |
|University of Illinois at Chicago   Internet: U14663 at uicvm.uic.edu |
|Department of Oral Medicine         Fax: 1-312-996-1022            |
|  and Diagnostic Sciences           Phone: 1-312-996-8630          |
|801 S. Paulina                                                     |
|Chicago, IL 60612                                                  |

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net