>Can anyone give me a source for blackened, gridded, 0.22um, mixed ester
>membrane filters for bacterial enumeration techniques?
>I can find 0.45 um filters but not 0.22 um.
>Dye them yourself using irgalan black. For epifluorescence microscopy we
use 0.22 =B5m polycarbonate (nucleopore) filters which have previously been
stained overnight with irgalen black (0.2% in 2% acetic acid) and rinsed in
particle-free water. You can use other makes of filters (in fact, mixed
ester filters stain better), but nucleopores are the flattest and best for
microscopy. You shouldnt need a grid on the filter for microscopy, or are
you using them for cultural techniques?
John W. Patching
The Martin Ryan Marine Science Institute
University College Galway, Ireland
Email: John.Patching at UCG.IE
Phone: +353-91-524411 Ext 2398
=46ax: +353-91-750456 or +353-91-525005
