hosts for pMal-p vectors

MertensR at JRF.be MertensR at JRF.be
Fri Mar 15 10:12:44 EST 1996


Does anyone know if there are better expression hosts for the bacterial 
(Escherichia coli) expression vector pMal-p (from New England Biolabs) 
than the TB1 strain?

Did anyone try a protease-deficient strain?

I'm expressing G protein coupled receptors and the yield is pretty low 
(40 fmol/1000000000bacteria). If someone knows how to boost the 
production please let me know. (I have tried to change the OD600 for 
induction and the growth time after induction and the temperature...)

Any suggestions are more than welcome!

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