<<Subject: HELP - Selective yeast media
From: Mark Fuller <mark_fuller at qmgate.anl.gov>
Date: 15 Mar 1996 17:36:52 GMT
<<Does anyone know of a quick and easy media to grow/select for yeasts and
inhibit bacterial growth? Maybe even something you don't have to order
from Difco (recipe please).>>
The problem is over growth of molds. Antibiotics and acid (tartaric 10%
sterile add to make 3.5 pH) will easily inhibit bacteria, see PDA, DG18,
FDA-BAM Chapter 19, USP-MLT, etc. I suggest "Rose Bengal-dichloran- with
20% sucrose-agar-medium with added chlortetracycline (it's better than
chloramphenicol IMO). Or, a combination of chlortetracycline and
streptomycin is good. See OXOID (not DIFCO). This medium inhibits
bacteria and molds.
Contact me for more info. See my web page too.
<<Also, would a gram stain of a yeast look like HUGE, ovoid gram positive
rods/coccobacilli?>>
Yes, that is right.
<<Mark Fuller, Ph. D.
Environmental Research Division € ER/203-J070
Argonne National Laboratory
Argonne, IL 60439-4843>>
<<phone: (708) 252-1795
FAX: (708) 252-8895
email: mark_fuller at qmgate.anl.gov>>
