help in growing actinomycetes

Oliver Politz droli at biobase.dk
Wed Mar 20 11:35:16 EST 1996

Dear Netters
I need some help to grow the actinomycete Microbispora bispora.
I can grow it on oat meal agar as it is recommended by the ATCC 
culture collection. But because I want to isolate genomic DNA I have to 
grow it in a liquid cultuer. Up to now without any success.
My questions are. Wich medium is the best for this strain?
What  is the generation time and what is a good and fast method to 
isolate genomic DNA?
Thanks  in advance
PS. Please feel free to email directly to 
droli at biobase.dk

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