Hello netters
I have read (I had the paper in my hands) that the relation between Kan
resistance (gen from Tn5) and expression of the kan gene is "linear" when
the medium is buffered. I think that the authors of that paper use this
system to select kan gene recombination from the chromosome to a
multicopy plasmid.
But I cannot remember nor the author, nor the journal, anything from that
paper. I have mahe extensive searches through several databases, Current
Contents and so, without succes; besides is really difficult because I
don't remember the year either.
So... would you please give me any information about that issue you can
have on hand? It would be very useful.
TIA,
Rafa
___________________________________________________________________
|
Rafael Maldonado | 'No te creas todo
room 6160 Eccles Institute of Human Genetics | lo que leas en
Department of Human Genetics | internet.'
University of Utah |
Salt Lake City, Utah 84112. USA. | "Don't believe
Rafael.Maldonado at genetics.utah.edu | everything you read
Rafael at howard.genetics.utah.edu | in internet."
Tel: 801-581-4429 |
Fax: 801-585-3910 |
