I have just conducted some experiments to determine the minimum
inhibitory concentration (MIC) of a chemical and a bunch of different
bacteria. I have data covering the initial compound concentrations, the
optical density (OD) over time of the cultures and the final compound
and metabolite concentrations.
1) What should be graphed versus what? Initial concentration vs. OD
gives a good trend, but is there any model/equation I can use to figure
out the MIC? I can eyeball it, but I'd much rather do it
mathematically.
2) What's the general feeling of reporting LC-50, EC-50 along with MIC
values?
Any input or hints on specific references would be much appreciated.
-Mark Fuller, Ph.D.
