Mark Fuller wrote:
>> I have just conducted some experiments to determine the minimum
> inhibitory concentration (MIC) of a chemical and a bunch of different
> bacteria. I have data covering the initial compound concentrations, the
> optical density (OD) over time of the cultures and the final compound
> and metabolite concentrations.
>> 1) What should be graphed versus what? Initial concentration vs. OD
> gives a good trend, but is there any model/equation I can use to figure
> out the MIC? I can eyeball it, but I'd much rather do it
> mathematically.
>> 2) What's the general feeling of reporting LC-50, EC-50 along with MIC
> values?
>> Any input or hints on specific references would be much appreciated.
>> -Mark Fuller, Ph.D.
Mark,
In Excel 5, there's a tool to have a mathematical representation of
the graph created by the variables chosen. You can play around with it
to determine an equation.
Nathaniel Sopelario
