Nathaniel Sopelario (nate at netspace.net.au) wrote:
: Mark Fuller wrote:
: >
: > I have just conducted some experiments to determine the minimum
: > inhibitory concentration (MIC) of a chemical and a bunch of different
: > bacteria. I have data covering the initial compound concentrations, the
: > optical density (OD) over time of the cultures and the final compound
: > and metabolite concentrations.
: >
: > 1) What should be graphed versus what? Initial concentration vs. OD
: > gives a good trend, but is there any model/equation I can use to figure
: > out the MIC? I can eyeball it, but I'd much rather do it
: > mathematically.
: In Excel 5, there's a tool to have a mathematical representation of
: the graph created by the variables chosen. You can play around with it
: to determine an equation.
Alternatively, you could use the well-established procedures of NCCLS to
determine MIC.
Candy Krepel
Surgical Microbiology Research Lab, Medical College of Wisconsin
ckrepel at post.its.mcw.edu
