The reference to Prusiner brought back memory of his article which
appeared in Science in 1982 where a lot is said about the resistance of
prion to a number of things. The reference is: Science Vol. 216, No.
4542 (9 April 1982):136-144.
Department of Biological Sciences
Clinton, MS 39058
snazelle at mc.eduhttp://www.mc.edu/~snazelle/
>>> Oladele A. OGUNSEITAN <oaogunse at uci.edu> 03/28/96 01:08pm
You may find answers to your questions if you read up on
Stanley Prusiner's (UC-Berkeley) work over the past 30 years or so on
prions. He answered many of these question through work on the
sheep variety of prion that has been around for a long time. Many
people still cling to the unproven idea that a nucleic acid is somehow
involved in the stability of the apparent protein.
University of California
On 28 Mar 1996, Nicholas Landau wrote:
> > I am a microbial ecologist, but this BES/CJS connection has caught
> my attention, just like everybody else.
> > I have noticed postings from UK microbiologists attesting to the
> great heat resistance of the BSE infective agent. I have two
> questions about this.
> > 1) Is there any hyposthesis as to how a protein, such as the BSE
> prion, could retain its secondary and tertiary structure? Secondary
> structure is generally determined by hydrogen bonds, which are
> at around 100 C in most substances. How is it that BSE can retain its
> functionality after exposure to normally denaturing temperatures?
> Does it have a high cystein content?
> > 2) Given that the symptoms of BSE take many years after infection
> to manifest themselves, how is the virulence of the infective agent
> determined after such treatment? Have the experiments been going on
> for years and decades, or is some criterion other than the causation
> of symptoms in test subjects used to determine virulence?
> > Oh, yeah: one more question not involving thermostability....
> > 3) From the postings I have seen, it looks as if the infective
> agent has not been isolated and identified (some people say it is
> a virus, others a prion.) This being the case, how is it that
> the British government can conclude that CJS is being caused by
> the same infectious agent as BSE?
> > Thank you for responding.
> > Nick Landau
> Dept. Biochemistry and Microbiology
> Rutgers University
>nlandau at eden.rutgers.edu> >