Accessible via http://gepasi.dbs.aber.ac.uk/roy/chemom.htm:
Identification of oral abscess bacteria from hospital patients - Goodacre, R.,
Hiom, S.J., Cheeseman, S.L. Murdoch, D., Weightman, A.J. & Wade, W.G. (1996)
Identification and discrimination of oral asaccharolytic Eubacterium spp. by
pyrolysis mass spectrometry and artificial neural networks. Current
Microbiology 32, 77-84.
ABSTRACT:
Curie-point pyrolysis mass spectra were obtained from 29 oral asaccharolytic
Eubacterium strains, and 6 abscess isolates previously identified as
Peptostreptococcus heliotrinreducens. Pyrolysis mass spectrometry (PyMS) with
cluster analysis was able to clarify the taxonomic position of this group of
organisms. Artificial neural networks (ANNs) were then trained by supervised
learning (with the back-propagation algorithm) to recognise the strains from
their pyrolysis mass spectra; all Eubacterium strains were correctly identifi
ed and the abscess isolates were identified as un-named Eubacterium taxon C2
and were distinct from the type strain of P. heliotrinreducens. These results
demonstrate that the combination of PyMS and ANNs provides a rapid and
accurate identification technique.
Roy
Dr Roy Goodacre
E-mail: rrg at aber.ac.ukhttp://gepasi.dbs.aber.ac.uk/roy/rgcv.htm
Voice : +44 (0)1970 621947 Fax: +44 (0)1970 622354
Snail : Inst Biol Sci, Univ Wales, Aberystwyth, Dyfed SY23 3DA, WALES