Hello!
In lab. I isolated & amplified a segment of the TPA gene that was poly-
morphic for an Alu insertion. The two amplified allele were 400 bp (conatining
an Alu insert), and 100 bp (without an insert).
1) I want to know the exact sequences of these two amplified alleles ?
2) How long are these sequences including the extended primers ?
3) Is there an Alu restriction site in any of these sequences ? & Where?
4) The map of the 400 and 100 bp alleles (i.e. which common restriction sites
are present, how often they cut and where they cut).
Please, send this info directly to: kabakibi at pegasus.montclair.edu
Thank you.
