Hi, Jane.
If you are simply trying to look at the lysozyme activity, the standard
system is to measure the rate of breakdown of cell walls of Micrococcus
lysodeikticus. The cell walls of this organism are exquisitely sensitive
to lysozyme activity. You measure the rate of decrease in optical density
at 450 nm, using a hen egg lysozyme to make a standard curve. Protocols
should be easy to find.
Hope this helps!
Patti
On Tue, 5 Nov 1996, Jane Allen wrote:
> I would like some suggestions on what bacteria I can use to do an
> experiment on the lysosomal property of tears. Any other suggestions on
> this experiment would be welcome.
> Thank you in advance
> Jane
>>
