Michael:
Bacteriophages infecting Pseudomonas putida are very rare.
Several years ago, A.M. Chakrabarty constructed or found a "shuttle"
phage that can infect both P. aeruginosa and P. putida. The reference I
have is as follows:
Chakrabarty, A.M. and I.C. Gunsalus. 1970. Transduction and Genetic
Homology Between Pseudomonas species putida and aeruginosa. Journal of
Bacteriology. Volume 103, number 3, pages 830-832.
The name of the phages are pf16h2 and pf20.
I hope they still have them. Good luck!
O.A. Ogunseitan
Assistant Professor
Department of Environmental Analysis & Design
University of California
Irvine, CA 92697-7070
_______________________________________________________
On Fri, 8 Nov 1996, Michael Kertesz wrote:
> Hi,
>> I've been looking around in the literature for a generalized transducing
> phage which can be used in Pseudomonas putida to move
> chromosomal markers from one strain to another (an equivalent to P1 in E.
> coli, or F116 or G101 in P. aeruginosa). So far I have come up with
> almost nothing. Does anyone know of such phages, or haven't they been
> reported for putida?
>> thanks a lot
>>> Michael Kertesz
>> ETH-Microbiology,
> ETH-Zentrum/LFV,
> CH-8092 Zurich, Switzerland
>> tel: +41-1-632 3357
> fax: +41-1-632 1148
> e-mail: kertesz at micro.biol.ethz.ch>>
