P. putida transduction

Oladele A. OGUNSEITAN oaogunse at UCI.EDU
Fri Nov 8 14:39:42 EST 1996


	Bacteriophages infecting Pseudomonas putida are very rare.  
Several years ago, A.M. Chakrabarty constructed or found a "shuttle" 
phage that can infect both P. aeruginosa and P. putida.  The reference I 
have is as follows:

Chakrabarty, A.M. and I.C. Gunsalus.  1970.  Transduction and Genetic 
Homology Between Pseudomonas species putida and aeruginosa.  Journal of 
Bacteriology. Volume 103, number 3, pages 830-832.

The name of the phages are  pf16h2  and pf20.  

I hope they still have them.  Good luck!

O.A. Ogunseitan
Assistant Professor
Department of Environmental Analysis & Design
University of California
Irvine, CA 92697-7070

On Fri, 8 Nov 1996, Michael Kertesz wrote:

> Hi,
> I've been looking around in the literature for a generalized transducing 
> phage which can be used in Pseudomonas putida to move 
> chromosomal markers from one strain to another (an equivalent to P1 in E. 
> coli, or F116 or G101 in P. aeruginosa). So far I have come up with 
> almost nothing. Does anyone know of such phages, or haven't they been 
> reported for putida?
> thanks a lot
> Michael Kertesz
> ETH-Microbiology,
> ETH-Zentrum/LFV,
> CH-8092 Zurich, Switzerland
> tel: +41-1-632 3357
> fax: +41-1-632 1148
> e-mail: kertesz at micro.biol.ethz.ch

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