R.C.Ogden wrote:
>> Our lab has now regularly sequences degradative genes (mainly from
> Pseudomonas sp.). We would like to identify any upstream regulatory
> sequences, or potential regulatory sequences, such as promoters, known
> prokaryotic enhancers and regulator binding sites (such as those for LysR
> and NtrC regulator families).These seems to be several programs and web
> sites offering similar analysis of EUKARYOTIC sequence data but very
> little aimed at E.coli and other bacteria. Can anyone suggest any
> resources to scan our upstream sequence ?
I believe you could use the help of the Baylor College of Medicine (or
more specifically, the BCM Search Launcher). They are found on-line at
the following:
http://gc.bcm.tmc.edu:8088/search-launcher/launcher.html
Good Luck!!
C. J. Fields
University of North Texas
cfields at gab.unt.edu
P.S. I will keep you in mind. Our lab here works with all kinds of
pseudomonads (most now reclassified). I believe that the promoters
aren't much different from E. coli, but I may be wrong (we express P.
putida and P. aeruginosa genes in E. coli all the time).
