Hello everybody,
I have been trying (without success) to do transformation/conjugation in a
strain of the bacteria Xanthomonas oryzae with a genomic library
(cosmid+insert size varying from 20-40 kb). So far, I'm only able to do
tranformation/conjugation when I use the vector without no insert(cosmid
size of 13.2 kb). The only time I thought I had got a sucessful
transformation with a cosmid+insert, after doing the prep and running the
gel, guess what? It had lost the insert!!
If anyone out there has any experience with Xanthomonas(or related
bacteria) I would very much appreciate any suggestions or helpfull
hints!!
Thank you in advance.
FRANCISCO GOES DA SILVA
Department of Plant Pathology
354 Hutchinson Hall
University of California, Davis
Davis, CA 95616
Phone: 916-752-7257
Fax: 916 - 752-5674
e-mail: fdgoesdasilva at ucdavis.edu
