Gary Lum <glum at ozemail.com.au> wrote:
>hsb30 at aol.com wrote:
>> Hello, I am trying to find out which lab test will identify an organism
>> "very specifically" as being Strep f. Anybody, please help. I need
>> to know ASAP.....
>> Please Email me at HSB30 at aol.com>> Thank you...
>>From memory you need PYR (pyrollidonyl arylamidase) to ensure it's an
>_Enterococcus_ (note change in genus name).
>>Make sure it's non-motile (use a Craigie's tube in semisolid BHI) and
>non-pigment producing (grow on clear media and use a swab to pick up
>colonies-pigment will show on the swab).
>>A positive sodium pyruvate test will make it 'faecalis' rather than
>'faecium'. Note that automated systems are not very good at identifying
>enterococci to a species level.
>>This is a rough scheme. I advise to visit your local clinical
>microbiology laboratory (local hospital) and ask to read the ASM's
>Manual of Clinical Microbiology.
>>Hope this helps.
>>Gary
>>>--
>********************************************************
>Dr Gary Lum
>Director of Microbiology
>Royal Darwin Hospital
>>Microbiologists do it with culture and sensitivity
>Meet me at http://www.ozemail.com.au/~glum/index.html>E-mail me at glum at ozemail.com.au or gary.lum at nt.gov.au
Gary,
I just had an unknown contaminent Identified as Enterococcus faecalis
using an MIDI system. The result was a .783 for faecalis. This is a
very good to high match. It may be the new software we have installed
but I can say that this is a significant improvement over the last
year in identifying Enterococcus at the species level.
Cheers,
Ian
