Help on growing SRB on agar plates

Joseph E. Mayer Jr mayer at sluvca.slu.edu
Thu Nov 28 00:45:31 EST 1996

In message <Pine.GSO.3.95.961123111749.15450A-100000 at design.eng.uci.edu> -
Arul Jayaraman <arul at design.eng.uci.edu>Sat, 23 Nov 1996 11:22:25 -0800
:>I am working with sulfate-reducing bacteria (Desulfovibrio vulgaris and D.
:>gigas). I am having difficulty in growing these bacteria on agar plates/
:>solid medium. I have tried growing them on Modified baar's medium agar
:>plates (the medium that ATCC prescribes for growing these bacteria in
:>liquid cultrues) and on Iverson Medium plates (Trypticase soy agar,
:>magnesium sulfate and sodium lactate). I incubate the plates in a gasPak
:>chamber (hydrogen + carbondioxide environment). But growth is very poor. I
:>appreciate it if anyone can give me suggestions/pointers on growing SRB on
:>Thank you
:>Arul Jayaraman
:>Dept. of Chemical & Biochemical Engineering
:>University of California at Irvine
:>Irvine, CA 92697-2575
:>Ph : (714)-509-1060 (H)
:>     (714)-824-8389 (L)
:>Try washing the agar several times in cold water. To this just add agar to a large breaker, add an aliqout of cold water and stir. After a few minutes filter the agar and repeat the process 6 times or so. After the last filtration step, I usually put the agar on some paper towels and let the agar dry. Store excess agar for later use. This removes organic matter that inhibits many lithotrophs. Hope this helps.


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