Armand M. Obiang wrote:
>> Could you say me in what conditions I would get Mycobacterium ulcerans
> liquid culture. This mycobacterium is a very slow grower which needs 3-4
> weeks to show colonies on LJ medium (egg base medium). I want to purify
> Mycobacterium ulcerans exotoxin and I have failed to subculture
> Mycobacterium ulcerans grown on LJ medium, in Sauton medium, Dubos medium
> + 5 % BSA + 7.5 dextrose, MIDDLEBROOK 7H9 + ADC. THANS IN ADVANCE TO ALL.
>
You can also try RVb10 (Redmond and Ward, Bull. WHO, 35:563-568, 1966).
Seems to be better than 7H9.
I hope it helps.
--
***** JOAO VASCONCELOS COSTA, MD, PhD
***** Instituto Gulbenkian de Ciencia - Oeiras, Portugal
***** mailto:jcosta at pen.gulbenkian.pt
***** http://www.pen.gulbenkian.pt/v2/jvc.html
