Sequencing 7-8 kb deletion

Sharon Jean Kelley skelle2 at unix1.sncc.lsu.edu
Wed Oct 9 11:42:05 EST 1996


I wish to sequence a large deletion to determine its break points.  I 
know from restriction analysis that a 13 kb region of lambda DNA has been 
reduced to 5 kb.  Any advice on how to tackle this problem would be 
appreciated.  I have experience with cycle sequencing using S35 labeled 
dATP.  I do not have access to an automated sequencer.

Thanks in advance for any advice.


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