I have to induce beta-galactosidase in a liquid culture of E.coli Y1089
lysogen for a lambda gt11 phage containing a beta-galactosidase-fusion
protein gene.
I have read in a protocole to use 10 mM IPTG, which seems to me an
enormous amount, since I have to make a liter scale culture.
Is this concentration required? What is the minimal efficient
concentration to fully induce beta-galactosidase synthesis?
--
Daniele EUSEBE(Ecole Normale Supérieure-INSERM U293-Paris-France)
e-mail:deusebe at wotan.ens.fr
