Dear Jose:
jose.mejia at well.ox.ac.uk wrote:
> I need to know if there are any aminoglycosides inactivated by the type I
> phosphotransferase encoded on Tn903, which are not inactivated by the type
> II enzyme from Tn5. Or the reverse.
Check out the following review:
Shaw KJ, Rather PN, Hare RS, Miller GH. 1993. "Molecular genetics of
aminoglycoside resistance genes and familial relationships of the
aminoglycoside-modifying enzymes." Microbiological Reviews 57(1):138-163
According to it, the gene from Tn903 is classified as aph(3')-Ia and provides
resistance to kanamycin, neomycin, paromomycin, ribostamycin, lividomycin, and
gentamicin B. The gene from Tn5 is classified as aph(3')-IIa and provides
resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and
gentamicin B. So it would seem that lividomycin and butirosin might distinguish the
two enzymes.
Cheers,
- Peter
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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England
Tel (01223) 402104 (international calls +44-1223-402104)
Fax (01223) 402140 ( " " +44-1223-402140)
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