S. mutans will metabolize sugars to acids, primarily lactic. This is
the basis of it's putative role in dental caries. You may not be
successful trying to find a buffer strong enough to overcome the aicd
production.
Have you considered continuous culture techniques?
Scott Sutton, PhD
The Microbiology BBS
telnet: microbiol.org
http://microbiol.org
JX>Hi, there,
JX> I'm trying to maintain pH value at 7.0 or 5.5 to culture
JX>S.mutans GS-5. I used PBS. But it doesn't work. After o/n, the pH
JX>value drops down too much. Anybody out there knows which buffer
JX>I should use, please reply to this account. Thanks a lot in
JX>advance.
JX>Jinling Xie
