Antimicrobial Susceptibility Test For Pneumococci

Sysop sysop at org
Tue Oct 22 04:58:12 EST 1996

I am sorry but I have no practical experience with the Spiral Plater.
However, you can find contact information on the web at
http://microbiol.org/mbbsorc.htm.  You might also try posting the
question on the Pharm. Microbiology Forum mail list (after joining, of

Scott Sutton, PhD
The Microbiology BBS
telnet: microbiol.org

HK>Hi all,

HK>Spiral plater endpoint method is a efficient and reproducible alterative
HK>methods of performing agar dilution susceptibility test. The spiral plater
HK>is used to create a concentration gradient of an antimicrobial agent
HK>that decreases in the agar as deposition progresses from near the center of
HK>the concentration gradient. After incubation, endpoint MIC are determined by
HK>measuring the distance between the center of the disk to the point where
HK>the growth of bacteria crease.

HK>I am now conducting a project on the use of spiral plater to determine the
HK>susceptibility of pneumococci to penicillin. However, I do not get the revis
HK>standard procedure and the revised formula other than that provided by the
HK>prelimenary user guide of the spiral plater for the calculation of stock
HK>antibiotics required and the endpoint concentration on the agar.

HK>Moreover, do you have any idea on the use of this method to determine
HK>the MIC of pneumococci on various drug?

HK>Thank For Any Help.


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