IUBio

IPTG concentration for induction?

Diane E. Emery emery at bcm.tmc.edu
Thu Oct 24 18:36:09 EST 1996


In article <541cn3$ern at chopin.inet.co.th>, Umnarj Paeratakul
<umnarj at mozart.inet.co.th> wrote:

> Dear friend
> 
> Yes it is a surprise for me too!  I looked up IPTG concentration 
> from Jeffrey Miller book, Experiments in molecular genetics,
> Cold spring Harbor, and it also said 10 mM final concentration.
> 
> Umnarj Paeratakul
> Thailand


We routinely use IPTG at 1mM, although I have seen others use it as low as
0.5mM.

Diane



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