Q: how to maintain pH value to culture Streptoccus mutans GS-5?

Robert Randolph rand777 at ids.net
Sun Oct 27 16:32:57 EST 1996

On 20 Oct 1996 18:51 EST, jxie at ubvms.cc.buffalo.edu (Jinling Xie)

>Hi, there,
>    I'm trying to maintain pH value at 7.0 or 5.5 to culture
>S.mutans GS-5. I used PBS. But it doesn't work. After o/n, the pH
>value drops down too much. Anybody out there knows which buffer
>I should use, please reply to this account. Thanks a lot in 
>Jinling Xie

A method used in the past, but seldom mentioned today is the addition
of marble chips to the medium prior to sterilization.  I have
successfully used this method in large volume bioreactor procedures.
R.Randolph - rand777 at ids.net
Robert Randolph
Newport, RI
rand777 at ids.net

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