Haishan Xiong <hxx101 at email.psu.edu> wrote:
>I have been experiencing some problems with growing E. coli in M9
>minimum media. I tried to grow JM109 and XL1-Blue cells in M9 +
>glucose. Neither grew. I did not find any difficiency in terms of
>their genotype. How can I get around with this problem?
>I appreciate your kindly help.
>Haishan
Did you include thiamine? Both those strains require it. I make and
filter-sterilize a 3.4 mg/ml stock solution (of thiamine
hydrochloride) which is 200x. I add it, along with glucose and other
supplements, after autoclaving and cooling the media (and for plates,
just before pouring). I store the stock solution frozen.
Hope this helps!
Bruce Amsden
