When much proteins of large molecular weight co-exist, do proteins of
small molecular weights(6500 dalton) tend to be underestimated on
SDS-PAGE?
In other words, compared to their absolute contents their bands look
light in
mixture of very much of other proteins but when only they are SDS-PAGEd
their bands look dark despite of the same contents.
Is it possible?
Any comments and any reference will be appreciated.
Thank you.
