Are small proteins underestimated on SDS-PAGE?

Achim Recktenwald, PhD achim at ibex.ca
Wed Jan 8 07:57:40 EST 1997

Lee, Ji Hyun wrote:
> When much proteins of large molecular weight co-exist, do proteins of
> small molecular weights tend to be underestimated on SDS-PAGE? In other
> words, compared to their absolute contents their bands look light in
> mixture of very much of other proteins but when only they are SDS-PAGEd
> their bands look dark despite of the same contents.
> Is it possible? What reference is helpful to me?
> Any comments will be appreciated.
> Thank you.

Though I cannot give you a literature reference, from my own experience
I think this to be true.


More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net