We use M. smegmatis in our microbiology course for both cultural
characteristics and to teach acid-fast staining. We have never had to
vary growing conditions from those of the environment. M. Smegmatis can
be included as part of the "normal flora" in terms of its growth
requirements, so try growing it at 37 degrees on TSA or in TSB. For
further information, consult Bergey's Manual 9th ed. I hope this helps.
Karl J. Roberts
Assistant Professor of Biology
Prince George's Community College
On Tue, 14 Jan 1997, M. D. Unson wrote:
> Hello all,
> I need information on what percent oxygen to use for growing nonpathogenic
> mycobacteria (I use M. smegmatis). Do they prefer a lower O2 tension than
> that of atmospheric levels? Also, do they like added CO2? Most information
> I have found is for pathogens like TB.
> Please respond to me privately as I don't often read the newsgroup.
> Thanks to all in advance,
>munson at ucsd.edu>