Does anyone know what the minimum amount of 16SrRNA (or rDNA) sequence
needed to make a BACTERIAL species designation ?
I have two examples in mind.
1) For example, the 16S rRNA sequence does not match any previously known
16S rRNA sequence, and it looks like a new species
... for scenario #1, how much of the total 16S rRNA must be sequenced
before one can conlude that it is a new species
2) For example, a partial 16S rRNA sequence obtained is a 99% match with an
existing sequence in the database, but only a portion of the 16S rRNA has
been sequenced.
... for scenario #2, how much must be sequenced (assume that the sequence
will contain parts of 16S rRNA molecule that are variable) to allow you to
make a specific species identification of a bacterium
Thank you for any feedback.
Any good sources of information on this subject would also be appreciated
(i.e books, review articles, etc.)
-David
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