oxidative stress

MARK PEGGIE mwpeggie at biolsci.dundee.ac.uk
Fri Jan 17 06:51:27 EST 1997

You could try diamide (around between 10-500uM).  The only problem is that it 
seems to be pretty unstable.  (Kosower & Kosower, Nature 224 1117-1120, 1969)

In article <671687537D at rose.le.ac.uk> mdg at leicester.ac.uk (Dr Martin Goldberg) 
writes:>From: mdg at leicester.ac.uk (Dr Martin Goldberg)
>Subject: oxidative stress
>Date: 11 Jan 1997 06:29:29 -0800

>>To: microbio at net.bio.net

>>From: Dr Wangxue Chen <wakefiel at ihug.co.nz>

>>Subject: Oxidative stress of bacteria

>>Date: 4 Jan 1997 06:24:19 GMT

>>I would like to give oxidative stress to bacteria (such as E. coli)
>>cultured either on agar plate (preferred) or in liquid medium. I know
>>someone use hydrogen peroxide or other chemicals. Any advice or
>>protocols would be very appreciated.



>I understand that you can add menodione to the culture medium. I'm
>afraid that I don't know what concentration to use, but if you try
>searching the literature databases, using the keywords "menodione" and
>"oxidative stress", you should get your answer.


>Martin Goldberg
>* Dr Martin Goldberg,                   *
>* Dept. of Microbiology and Immunology, *
>* University of Leicester,              *
>* University Road,                      *
>* LEICESTER.                            *
>* LE1 9HN                               *
>* UK                                    *
>* Tel. +44 (0)116-252 3017              *
>* Fax. +44 (0)116-252 5030              *
>* E-mail mdg at le.ac.uk                   *

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