You could try diamide (around between 10-500uM). The only problem is that it
seems to be pretty unstable. (Kosower & Kosower, Nature 224 1117-1120, 1969)
In article <671687537D at rose.le.ac.uk> mdg at leicester.ac.uk (Dr Martin Goldberg)
writes:>From: mdg at leicester.ac.uk (Dr Martin Goldberg)
>Subject: oxidative stress
>Date: 11 Jan 1997 06:29:29 -0800
>>To: microbio at net.bio.net
>>From: Dr Wangxue Chen <wakefiel at ihug.co.nz>
>>Subject: Oxidative stress of bacteria
>>Date: 4 Jan 1997 06:24:19 GMT
>>I would like to give oxidative stress to bacteria (such as E. coli)
>>cultured either on agar plate (preferred) or in liquid medium. I know
>>someone use hydrogen peroxide or other chemicals. Any advice or
>>protocols would be very appreciated.
>>Thanks
>>Chen
>I understand that you can add menodione to the culture medium. I'm
>afraid that I don't know what concentration to use, but if you try
>searching the literature databases, using the keywords "menodione" and
>"oxidative stress", you should get your answer.
>Regards,
>Martin Goldberg
>*****************************************
>* Dr Martin Goldberg, *
>* Dept. of Microbiology and Immunology, *
>* University of Leicester, *
>* University Road, *
>* LEICESTER. *
>* LE1 9HN *
>* UK *
>* Tel. +44 (0)116-252 3017 *
>* Fax. +44 (0)116-252 5030 *
>* E-mail mdg at le.ac.uk *
>*****************************************
