killing of Bacillus strains?

hbkg at hitel.kol.co.kr hbkg at hitel.kol.co.kr
Thu Jan 16 22:38:13 EST 1997

We have been trying to clone a protease gene from a Bacillus strain
to E. coli on the medium containing skim milk. Problems are that
it got often contaminated by some Bacillus.
What methods (including autoclaveing) do you think are most effective
in killing Bacillus spores?
Thank you in advance!!

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