Durham tubes and how do they work

Neal Machtiger neal_m at ix.netcom.com
Mon Jan 20 13:32:57 EST 1997

PRODEV1A wrote:
> I would like to know more about "Durham Tubes And how they work??"
Durham Tubes are used to detect gas formation resulting from bacterial
fermentation of carbohydrates.

The tubes are placed, inverted into culture tubes to which the substrate
containing medium is added, prior to autoclaving. The trapped air is
driven from the inverted Durham tube in the autoclave. As the autoclaved
tubes coll following remolval from the autoclave, the medium is trawn
into the Durham tube, filling it COMPLETELY.

THe tubes for subtrate containing medium are then inoculated and
incubated.If there is gas formations, usually CO2, the gas is trapped in
the inverted Durham tube, demonstrating gas formation.

Most baterial taxonomic schemes indicate if a particular specis uses a
specific substrate, and whether gas formation is expected.

More details should be ain any complete bacterial biochemistry book


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