Durham tubes and how do they work

hsamander at sprynet.com hsamander at sprynet.com
Wed Jan 22 20:24:59 EST 1997

>   PRODEV1A <PRODEV1A at fox.nstn.ca> writes:
>  I would like to know more about "Durham Tubes And how they work??"
In response to your question, a Durham tube is a test tube that contains an inverted vial..the formation of a bubble within this 
vial reveal gas production by the microbe being tested.  For example, "the fermentation of sugars by microorganisms is 
accompanied by the formation of either acid or acid and gas;  the presence of the acid is detected by a pH indicator in the 
medium.  [this works for sugar fermentation tests]  If you are testing for sugar, the medium will turn from red to yellow.  Again, 
gas production is determined by whether or not there is bubble formation within the vial.

	Many biochemical tests are done using test tubes, however in well-developed labs there are easier ways to do 
biochemical tests.  However, a few that can be done using test tubes are: mixed acid fermentation tests, Voges-Proskauer 
tests..these are biooxidation testing.   

I hope that is helpful to you..try reading a few lab manuals that discuss testing for pathogenic organisms..they will have
more extensive explainations!!!

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