Does anyone know the minimum number of bp needed for a double crossover
event at each end? Or an average range? I have a very small gene (200 bp
on each end, disrupted with an antibiotic cassette) that
won't transform. Of course the disruption could be lethal...
Thanks
David
David J. McGee
MCGEED at allegheny.edu
Graduate Student of Dr. Richard Rest
Snail mail:
Allegheny University of the Health Sciences
2900 Queen Lane
Dept. Microbiology and Immunology M.S. 410
G-68
Philadelphia, PA 19129
Phone: 215-991-8363
Fax: 215-848-2271