In article <5c6elb$ptg at juliana.sprynet.com>, hsamander at sprynet.com says:
>>> PRODEV1A <PRODEV1A at fox.nstn.ca> writes:
>> I would like to know more about "Durham Tubes And how they work??"
>>>>>>>>>>In response to your question, a Durham tube is a test tube that contains an inverted vial..the formation of a bubble within this
>vial reveal gas production by the microbe being tested. For example, "the fermentation of sugars by microorganisms is
>accompanied by the formation of either acid or acid and gas; the presence of the acid is detected by a pH indicator in the
>medium. [this works for sugar fermentation tests] If you are testing for sugar, the medium will turn from red to yellow. Again,
>gas production is determined by whether or not there is bubble formation within the vial.
>> Many biochemical tests are done using test tubes, however in well-developed labs there are easier ways to do
>biochemical tests. However, a few that can be done using test tubes are: mixed acid fermentation tests, Voges-Proskauer
>tests..these are biooxidation testing.
>>I hope that is helpful to you..try reading a few lab manuals that discuss testing for pathogenic organisms..they will have
>more extensive explainations!!!
Gadgets such as API and Micro ID and Enterotubes, and discs impregnated
with 'whatever substrate' are all relatively new --25-30 years ago
the inverted little tube was as common in the lab. as the petri plate.
For some types of work they are still the best way to go.