In article <32E05F88.596F at sprynet.com>, Louis Geller <bbbbd at sprynet.com> wrote:
[...]
* Now, we are trying to amplify a single plasmid of this library that
* contains a yeast gene we have cloned by complementation. The plasmid
* was removed from yeast and transformed into E. coli strain BMH.
* After 3 tries, we have been unable to get *any* yield whatsoever! I
[...]
Take some of your culture just before you do the prep and plate it out
on + and - amp plates just to make sure that these cells aren't losing
the vector.
--Gene Cutler
