Here is the problem. I am working with several isogenic strains of S.
aureus. One of the strains will often lose turbidity after 3 to 4 hours
growth in broth and eventually become completely clear. This clearing
occurs only after inoculation of fresh broth with an overnight broth
culture--never happens in broth when inoculating from a plate. Addition of
the lysate to spread plates of S. aureus, E. coli, and B. subtilis produce
'plaques'. Filtration of the lysate with a 0.45 micron pore filter
eliminates plaque formation. What's the story?
