Staphylococcus aureus lysis

Keith Warner kwarner at unccvm.uncc.edu
Sat Jan 25 10:18:41 EST 1997

Here is the problem.  I am working with several isogenic strains of S.
aureus.  One of the strains will often lose turbidity after 3 to 4 hours
growth in broth and eventually become completely clear.  This clearing
occurs only after inoculation of fresh broth with an overnight broth
culture--never happens in broth when inoculating from a plate. Addition of
the lysate to spread plates of S. aureus, E. coli, and B. subtilis produce
'plaques'. Filtration of the lysate with a 0.45 micron pore filter
eliminates plaque formation.  What's the story?

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