Staphylococcus aureus lysis

GS microHero at compuserve.com
Mon Jan 27 01:37:38 EST 1997

Matthew or Dawnita Nilles wrote:
> In article <kwarner-2501971023530001 at cha-nc9-34.ix.netcom.com>,
> kwarner at unccvm.uncc.edu (Keith Warner) wrote:
> > Here is the problem.  I am working with several isogenic strains of S.
> > aureus.  One of the strains will often lose turbidity after 3 to 4 hours
> > growth in broth and eventually become completely clear.  This clearing
> > occurs only after inoculation of fresh broth with an overnight broth
> > culture--never happens in broth when inoculating from a plate. Addition of
> > the lysate to spread plates of S. aureus, E. coli, and B. subtilis produce
> > 'plaques'. Filtration of the lysate with a 0.45 micron pore filter
> > eliminates plaque formation.  What's the story?
> Sounds like you found bacteriophage (viruses of bacteria) I don't know why
> you see plaques on E. coli and B. subtilis, phage are usually very host
> specific.
> Does anyone know if it could be Bdellovibrio (parasitic bacteria).
> Matt Nilles
> Dept. of Microbiol. and Immunol.
> Univ. of Kentucky

Doesn't sound like phage - 0.45 micron filter wouldn't take it out - at 
least not on mechanical filtration. Bdellovibrio - where would it come 
from? If it's in the staph strain you might expect it to wipe out the 
plate cultures too.

Graham Shepherd

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net