GST-fusion system question

Bart K. Kwieciszewski riverflo at u.washington.edu
Thu Jan 30 13:44:41 EST 1997

I am trying to purify GST-NS5A fusion proein, but I'm having extreme
difficulties getting a a high yield expression.  First the fusion protein
is unstable, and second, the NS5A protein by itself is very unstable.  I
would like to purify about 500 micrograms of the full length product from
1-2 liters of original culture, but I'm ending up with about 100.  HELP!

Bart K. Kwieciszewski
University of Washington
Regional Primate Research Center
Department of Microbiology
HSB H-331

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