Dr Martin Goldberg wrote:
> I have a strain with a pBR322-based plasmid and I want to cure the
> strain of this plasmid. Is there any way of achieving this without
> using agents such as ethidium bromide? I am considering
> competing-out
> the plasmid by transforming the strain with pBR322 or one of its
> other derivatives and selecting for this latter plasmid. Has anybody
> got an
> opinion on this approach or are there any more elegant methods?
> *****************************************
Martin,
Curing by plasmid incompatibility should work fine as long as you
have a good, unique marker for the second plasmid. Most, if not all, of
the curing procedures I know of are purely empirical (SDS,EtBr, etc.).
Incompatibility would probably be one of the mildest procedures you
could use.
Don
