Detection of HAV in environmental samples?

[cyc at glink.net.hk]

On 5 Jun 1997 DAILEYD at APSU01.APSU.EDU wrote:

> Greetings everyone,
> 
> I am in need of a test to ID Hepatitis A virus from water samples.  Is
> anyone aware of a rapid test for the detection of the virus or do I have
> to culture the virus?  Thanks in advance for any help.
> 
> Don Dailey, Ph.D.
> Dept. of Biology
> Austin Peay State University
> Clarksville, TN
> 
> 


Have you thought of using the reverse transcriptase PCR to detect for the
presence of the RNA virus.  I think this is the fastest way of analysing
your samples without culturing.

You just have to be careful in your preparation of your samples.

C.Y. Cheung
Microbiology,
University of Hong Kong.